Publications

2024

  • E. T. Wargent, M. A. Kępczyńska, M. H. Kaspersen, E. Rexen Ulven, J. R. S. Arch, T. Ulven, C. J. Stocker. Chronic administration of hydrolysed pine nut oil to mice improves insulin sensitivity and glucose tolerance and increases energy expenditure via a free fatty acid receptor 4-dependent mechanism. Br. J. Nutr. 2024, May 16:1-8. [ article] [pdf]

2021

  • K. V. Sørensen, M. H. Kaspersen, J. H. Ekberg, A. Bauer-Brandl, T. Ulven, K. Højlund. Effects of delayed-release olive oil and hydrolyzed pine nut oil on glucose tolerance, incretin secretion and appetite in humans. Nutrients 2021, 13, 3407. [article]

2020

  • K. V. Sørensen, S. S. Korfitzen, M. H. Kaspersen, E. Rexen Ulven, J. H. Ekberg, A. Bauer-Brandl, T. Ulven, K. Højlund. Acute effects of delayed-release hydrolyzed pine nut oil on glucose tolerance, incretins, ghrelin and appetite in healthy humans. Clin. Nutr. 2020, 40, 2169-2179. [article]

2018

  • A. H. Hansen, E. Sergeev, D. Bolognini, R. R. Sprenger, J. H. Ekberg, C. S. Ejsing, C. J. McKenzie, Christine; E. Rexen Ulven, G. Milligan, T. Ulven. Discovery of a potent thiazolidine free fatty acid receptor 2 agonist with favorable pharmacokinetic properties. J. Med. Chem. 2018, 61, 9534-9550.
  • M. Schilperoort, A. D. van Dam, G. Hoeke, I. G. Shabalina, A. Okolo, A. C. Hanyaloglu, L. H. Dib, I. M. Mol, N. Caengprasath, Y.-W. Chan, S. Damak, A. R. Miller, T. Coskun, B. Shimpukade, T. Ulven, S. Kooijman, P. C. N. Rensen, M. Christian. The GPR120 agonist TUG-891 promotes metabolic 1 health by stimulating mitochondrial respiration in brown fat. EMBO Mol. Med. 2018, e8047.
  • S. F. Gallego, K. Højlund, C. S. Ejsing. Easy, Fast, and Reproducible Quantification of Cholesterol and Other Lipids in Human Plasma by Combined High Resolution MSX and FTMS Analysis. Am. Soc. Mass Spectrom. 2018, 1, 34-41.

2017

  • S. V. F. Hansen, T. Ulven. Pharmacological tool compounds for the free fatty acid receptor 4 (FFA4/GPR120). Handb. Exp. Pharmacol. 2017, 236, 33-56.
  • E. Sergeev, A. H. Hansen, D. Bolognini, K. Kawakami, T. Kishi, J. Aoki, T. Ulven, A. Inoue, B. Hudson, G. Milligan. A single extracellular amino acid in Free Fatty Acid Receptor 2 defines antagonist species selectivity and G protein selection bias. Sci. Rep. 2017, 7, 13741.
  • A. H. Hansen, E. Sergeev, S. K. Pandey, B. D. Hudson, E. Christiansen, G. Milligan, T. Ulven. Development and characterization of a fluorescent tracer for the free fatty acid receptor 2 (FFA2/GPR43). J. Med. Chem. 2017, 60, 5638-5645.
  • M. H. Kaspersen, L. Jenkins, J. Dunlop, G. Milligan, T. Ulven. Succinct synthesis of saturated hydroxy fatty acids and in vitro evaluation of all hydroxylauric acids at FFA1, FFA4 and GPR84. MedChemComm 2017, 8, 1360-1365.
  • N. R. V. Dragano, C. Solon, A. F. Ramalho, R. F. de Moura, D. S. Razolli, E. Christiansen, C. Azevedo, T. Ulven, L. A. Velloso. Polyunsaturated fatty acid receptors, GPR40 and GPR120, are expressed in the hypothalamus and control energy homeostasis and inflammation. J. Neuroinflamm. 2017, 14, 91.
  • K. R. Watterson, S. V. F. Hansen, B. D. Hudson, E. Alvarez-Curto, S. Z. Raihan, C. M. G. Azevedo, G. Martin, J. Dunlop, S. J. Yarwood, T. Ulven, G. Milligan. Probe-dependent negative allosteric modulators of the long-chain free fatty acid receptor FFA4. Mol. Pharmacol. 2017, 91, 630-641.
  • J. M. Houthuijzen, I. Oosterom, B. D. Hudson, A. Hirasawa, L. G. M. Daenen, C. M. McLean, S. V. F. Hansen, M. T. M. van Jaarsveld, D. S. Peeper, S. Jafari Sadatmand, J. M. L. Roodhart, C. H. A. van de Lest, T. Ulven, K. Ishihara, G. Milligan, E. E.Voest. Fatty acid 16:4(n-3) stimulates a GPR120-induced signaling cascade in splenic macrophages to promote chemotherapy resistance. FASEB J. 2017, 31, 2195-2209.
  • G. Milligan, B. Shimpukade, T. Ulven, B. D. Hudson. Complex pharmacology of free fatty acid receptors. Chem. Rev. 2017, 117(1), 67-110. doi: 10.1021/acs.chemrev.6b00056.

2016

  • C. M. G. Azevedo, K. R. Watterson, E. T. Wargent, S. V. F. Hansen, B. D. Hudson, M. A. Kępczyńska, J. Dunlop, B. Shimpukade, E. Christiansen, G. Milligan, C. J. Stocker, T. Ulven. Non-acidic free fatty acid receptor 4 agonists with antidiabetic activity. J. Med. Chem. 2016, 59(19), 8868-8878.
  • L. Björkman, J. Mårtensson, M. Winther, M. Gabl, A. Holdfeldt, M. Uhrbom, J. Bylund, A. H. Hansen, S. Pandey, T. Ulven, H. Forsman, C. Dahlgren. The neutrophil response induced by an agonist for the free fatty acid receptor 2 (FFAR2/GPR43) is primed by the TNF-a and by receptor uncoupling from the cytoskeleton but attenuated by tissue recruitment. Mol. Cell Biol. 2016, 36(20), 2583-2595.
  • R. Prihandoko, E. Alvarez-Curto, B. D. Hudson, A. J. Butcher, T. Ulven, A. M. Miller, A. B. Tobin, G. Milligan. Distinct phosphorylation clusters determines the signalling outcome of the free fatty acid receptor FFA4/GPR120. Mol. Pharmacol. 2016, 89(5), 505-520.
  • E. Christiansen, B. D. Hudson, A. H. Hansen, G. Milligan, T. Ulven. Development and characterization of a potent free fatty acid receptor 1 (FFA1) fluorescent tracer. J. Med. Chem. 2016, 59(10), 4849-4858.
  • S. V. F. Hansen, E. Christiansen, C. Urban, B. D. Hudson, C. J. Stocker, M. E. Due-Hansen, E. T. Wargent, B. Shimpukade, R. Almeida, C. S. Ejsing, M. A. Cawthorne, M. U. Kassack, G. Milligan, T. Ulven. Discovery of a Potent Free Fatty Acid 1 Receptor Agonist with Low Lipophilicity, Low Polar Surface Area and Robust in Vivo Efficacy. J. Med. Chem. 2016, 59(6), 2841-2846.
  • M. Grundmann, I. G. Tikhonova, B. D. Hudson, N. J. Smith, K. Mohr, T. Ulven, G. Milligan, T. Kenakin, E. Kostenis, A molecular mechanism for sequential activation of a G protein-coupled receptor. Cell Chem. Biol. 2016, 23, 392-403.
  • D. Gozal, Z. Qiao, I. Almendros, J. Zheng, A. Khalyfa, B. Shimpukade, T. Ulven. Treatment with TUG891, a free fatty acid receptor 4 agonist, restores adipose tissue metabolic dysfunction following chronic sleep fragmentation in mice. Int. J. Obese. 2016, 40(7), 1143-1149.
  • S. V. F. Hansen, Z. E. Wilson, T. Ulven, S. V. Ley. Controlled generation and use of CO in flow. React. Chem. Eng. 2016, 1, 280-287.
  • M. E. Due-Hansen, S. Pandey, E. Christiansen, R. Andersen, S. V. F. Hansen, T. Ulven. A protocol for amide bond formation with electron deficient amines and sterically hindered substrates. Org. Biomol. Chem. 2016, 14, 430-433.
  • E. Sergeev, A. H. Hansen, S. K. Pandey, A. E. Mackenzie, B. D. Hudson, T. Ulven, G. Milligan. Non-equivalence of key positively charged residues of the free fatty acid 2 receptor in the recognition and function of agonist versus antagonist ligands. J. Biol. Chem. 2016, 291(1), 303-317.

2015

  • S. V. F. Hansen, T. Ulven. Oxalyl chloride as a practical carbon monoxide source for carbonylation reactions. Org. Lett. 2015, 17, 2832-2835. DOI: 10.1021/acs.orglett.5b01252.
  • T. Ulven, E. Christiansen. Dietary fatty acids and their potential for controlling metabolic diseases through activation of FFA4/GPR120. Annu. Rev. Nutr. 2015, 239-263.
  • E Christiansen, K. R. Watterson, C. J. Stocker, E. Sokol, L. Jenkins, K. Simon, M. Grundmann, R. K. Petersen, E. T. Wargent, B. D. Hudson, E. Kostenis, C. S. Ejsing, M. A. Cawthorne, G. Milligan, T. Ulven. Activity of dietary fatty acids on FFA1 and FFA4 and characterization of pinolenic acid as a dual FFA1/FFA4 agonist with potential effect against metabolic diseases. Br. J. Nutr. 2015, 113, 1677-1688.
  • E. Sokol, T. Ulven, N. J. Færgeman, C. S. Ejsing. Comprehensive and quantitative profiling of lipid species in human milk, cow milk and a phospholipid-enriched milk formula by GC and MS/MSALL. Eur. J. Lipid Sci. Technol. 2015, 117, 751-759.
  • G. Milligan, E. Alvarez-Curto, K. R. Watterson, T. Ulven, B.D. Hudson. Characterising pharmacological ligands to study the long chain fatty acid receptors GPR40/FFA1 and GPR120/FFA4. Br. J. Pharmacol. 2015, 172(13), 3254-3265.

2014

  • B. D. Hudson, E. Christiansen, H. Murdoch, L. Jenkins, A. H. Hansen, O. Madsen, T. Ulven G. Milligan. Complex pharmacology of novel allosteric Free Fatty Acid 3 Receptor ligands. Mol. Pharmacol. 2014, 86, 200-210.
  • B. D. Hudson, B. Shimpukade, G. Milligan, T. Ulven. The molecular basis of ligand interaction at free fatty acid receptor 4 (FFA4/GPR120). J. Biol. Chem. 2014, 289, 20345-20358.
  • A. J. Butcher, B. D. Hudson, B. Shimpukade, E. Alvarez-Curto, R. Prihandoko, T. Ulven, G. Milligan, A. B. Tobin. Concomitant action of structural elements and receptor phosphorylation determine arrestin-3 interaction with the free fatty acid receptor FFA4. J. Biol. Chem. 2014, , 289, 18451-18465.
  • K. R. Watterson, B. D. Hudson, T. Ulven, G. Milligan. Treatment of type 2 diabetes by free fatty acid receptor agonists. Front. Endocrinol. 2014, 5, 137.
  • E. Fjaere, U. L. Aune, K. Roen, A. H. Keenan, T. Ma, K. Borokowsky, D. M. Kristensen, G. W. Novotny, T. Mandrup-Poulsen T, B. D. Hudson, G. Milligan, Y. Xi, J. W. Newman, F. G. Haj, B. Liaset, K. Kristiansen, L. Madsen. Indomethacin treatment prevents high-fat diet-induced obesity and insulin resistance, but not glucose intolerance in C57BL/6J mice. J. Biol. Chem.2014 289, 16032-16045.
  • G. Milligan, T. Ulven, H. Murdoch, B. D. Hudson. G-protein-coupled receptors for free fatty acids: nutritional and therapeutic targets. Br. J. Nutr. 2014, 111, S3-S7.

2013

  • B. D. Hudson, B. Shimpukade, A. E. Mackenzie, A. J. Butcher, J. D. Pediani, E. Christiansen, H. Heathcote, A. B. Tobin, T. Ulven, G. Milligan. The pharmacology of a potent and selective agonist, TUG-891, demonstrates both potential opportunity and possible challenges to therapeutic agonism of FFA4 (GPR120). Mol. Pharmacol. 2013, 84, 710-725.
  • B. D. Hudson, M. E. Due-Hansen, E. Christiansen, A. M. Hansen, A. E. Mackenzie, H. Murdoch, S. K. Pandey, R. J. Ward, R. Marquez, I. G. Tikhonova, T. Ulven, G. Milligan. Defining the molecular basis for the first potent and selective orthosteric agonists of the FFA2 free fatty acid receptor. J. Biol. Chem. 2013, 288, 17296-17312.
  • E. Christiansen, S. V. F. Hansen, C. Urban, B. Hudson, E. T. Wargent, M. Grundmann, L. Jenkins, M. Zaibi, C. J. Stocker, S. Ullrich, E. Kostenis, M. U. Kassack, G. Milligan, M. A. Cawthorne, T. Ulven. Discovery of TUG-770: A highly potent free fatty acid receptor 1 (FFA1/GPR40) agonist for treatment of type 2 diabetes. ACS Med. Chem. Lett. 2013, 4, 441–445.
  • E. Sokol, R. Almeida, H. K. Hannibal-Bach, D. Kotowska, J. Vogt, J. Baumgart, K. Kristiansen, R. Nitsch, J. Knudsen, C. S. Ejsing. Profiling of lipid species by normal-phase liquid chromatography, nanoelectrospray ionization, and ion trap–orbitrap mass spectrometry. Anal. Biochem. 2013, 443, 88–96.
  • B. D. Hudson, H. Murdoch, G. Milligan. Minireview: The effects of species ortholog and SNP variation on receptors for free fatty acids. Mol. Endocrinol. 2013, 27, 1177-1187.
  • B. D. Hudson, T. Ulven, G. Milligan. The Therapeutic Potential of Allosteric Ligands for Free Fatty Acid Sensitive GPCRs. Curr. Top. Med. Chem. 2013, 13, 14-25.
  • E. Christiansen, M. E. Due-Hansen, C. Urban, M. Grundmann, J. Schmidt, S. V. F. Hansen, B. D. Hudson, M. Zaibi, S. B. Markussen, E. Hagesaether, G. Milligan, M. A. Cawthorne, E. Kostenis, M. U. Kassack, T. Ulven. Discovery of a potent and selective FFA1 agonist with low lipophilicity and high oral bioavailability. Med. Chem. 2013, 56, 982-992.

2012

  • B. Shimpukade, B. D. Hudson, C. K. Hovgaard, G. Milligan, T. Ulven. Discovery of a potent and selective GPR120 agonist. J. Med. Chem. 2012, 55, 4511-4515.
  • E. Christiansen, M. E. Due-Hansen, C. Urban, M. Grundmann, R. Schröder, B. D. Hudson, G. Milligan, M. A. Cawthorne, E. Kostenis, M. U. Kassack, T. Ulven. Free fatty acid receptor 1 (FFA1/GPR40) agonists: Mesylpropoxy appendage lowers lipophilicity and improves ADME properties. J. Med. Chem. 2012, 55, 6243-6644.
  • B. D. Hudson, E. Christiansen, I. G. Tikhonova, M. Grundmann, E. Kostenis, D. R. Adams, T. Ulven, G. Milligan. Chemically engineering ligand selectivity at the free fatty acid receptor 2 based on pharmacological variation between species orthologs. FASEB J. 2012, 26, 4951-4965.
  • T. Ulven. Short-chain free fatty acid receptors FFA2/GPR43 and FFA3/GPR41 as new potential therapeutic targets. Front. Endocrinol. 2012, 3, 111, doi:10.3389/fendo.2012.00111.
  • B. D. Hudson, I. G. Tikhonova, S. K. Pandey, T. Ulven, G. Milligan. Extracellular ionic locks determine variation in constitutive activity and ligand potency between species orthologs of the free fatty acid receptors FFA2 and FFA3. J. Biol. Chem. 2012, 287, 41195-41209.